6KWT

Crystal structure of Gre2 in complex with NADPH complex from Candida albicans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2019-05-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9796
Spacegroup name	I 2 3
Unit cell lengths	152.447, 152.447, 152.447
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.020
R-factor	0.1762
Rwork	0.172
R-free	0.21080
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.070
High resolution limit [Å]	3.020	3.020
Number of reflections	11745	592
<I/σ(I)>	32
Completeness [%]	100.0
Redundancy	41.1
CC(1/2)	0.999	0.815

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		280	0.1 M Sodium citrate tribasic dihydrate (pH5.6), 20% v/v 2-propanol, 20% w/v polyethylene glycol 4000