6KWS

Crystal structure of Gre2 from Candida albicans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-08-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9796
Spacegroup name	I 2 3
Unit cell lengths	149.594, 149.594, 149.594
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 2.800
R-factor	0.1762
Rwork	0.172
R-free	0.21080
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155: ???)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	13918	1384
<I/σ(I)>	43.8
Completeness [%]	100.0
Redundancy	10.4
CC(1/2)	0.998	0.949

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		280	0.1M Sodium citrate tribasic dihydrate (pH 5.6), 20%(v/v) 2-propanol, 20%(w/v) polyethylene glycol 4000