6KWH
Crystal Structure Analysis of Endo-beta-1,4-xylanase II Complexed with Xylotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2019-06-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.402, 58.633, 69.591 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.868 - 1.808 |
| R-factor | 0.1709 |
| Rwork | 0.168 |
| R-free | 0.21810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dfc |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.667 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.870 | 1.873 |
| High resolution limit [Å] | 1.808 | 1.808 |
| Rmerge | 0.121 | 0.121 |
| Number of reflections | 18405 | 1572 |
| <I/σ(I)> | 8.73 | |
| Completeness [%] | 98.3 | |
| Redundancy | 1.63 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4.5 | 291 | PEG 8000, NaI, NaAc-HAc |
