6KWA

AtDAO1(dioxygenase for auxin oxidation 1 from Arabidopsis thaliana)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-09-13
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 21 21 21
Unit cell lengths	76.626, 75.547, 165.318
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.881 - 2.094
R-factor	0.243821334415
Rwork	0.242
R-free	0.28150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5c3q 2brt
RMSD bond length	0.010
RMSD bond angle	1.375
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.160
High resolution limit [Å]	2.080	2.080
Rmerge	0.101	1.670
Number of reflections	56807	3898
<I/σ(I)>	11.7
Completeness [%]	96.4
Redundancy	7.2
CC(1/2)	0.998	0.472

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295.15	30mM KH2PO4, 16% PEG 8000, 20% glycerol