6KVR

Fatty acid amide hydrolase

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-02
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9897
Spacegroup name	P 1 21 1
Unit cell lengths	84.874, 68.753, 100.979
Unit cell angles	90.00, 99.63, 90.00

Refinement procedure

Resolution	28.110 - 2.200
R-factor	0.1726
Rwork	0.171
R-free	0.21760
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.925
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	58215	5814
<I/σ(I)>	32.4
Completeness [%]	99.8
Redundancy	7
CC(1/2)	0.900	0.899

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		280	100mM Tris/HCl (pH 8.5), 200mM Lithium sulfate, 30% w/v PEG 4000