6KVO

Crystal structure of chloroplast resolvase in complex with Holliday junction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 2
Unit cell lengths	121.316, 92.155, 65.894
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.020 - 2.500
R-factor	0.2035
Rwork	0.202
R-free	0.23430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.940
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14rc3_3206)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.030	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.252	0.506
Rmeas	0.275	0.549
Rpim	0.108	0.210
Number of reflections	26297	2898
<I/σ(I)>	5.3	2.2
Completeness [%]	99.8	98.9
Redundancy	6.5	6.6
CC(1/2)	0.954	0.891

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	Sodium acetate trihydrate, PEG 3350, Tacsimate