6KV0
Ferredoxin I from C. reinhardtii, high X-ray dose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-17 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 25.820, 32.300, 49.930 |
| Unit cell angles | 72.27, 78.09, 72.22 |
Refinement procedure
| Resolution | 28.718 - 1.400 |
| R-factor | 0.1757 |
| Rwork | 0.175 |
| R-free | 0.18740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.234 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.5.26) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.710 | 29.710 | 1.420 |
| High resolution limit [Å] | 1.400 | 7.540 | 1.400 |
| Rmerge | 0.101 | 0.066 | 0.439 |
| Rmeas | 0.117 | 0.077 | 0.508 |
| Rpim | 0.059 | 0.039 | 0.256 |
| Total number of observations | 679 | 5348 | |
| Number of reflections | 54425 | 185 | 1368 |
| <I/σ(I)> | 8.1 | 14.4 | 2.8 |
| Completeness [%] | 95.4 | 97.9 | 92.9 |
| Redundancy | 3.9 | 3.7 | 3.9 |
| CC(1/2) | 0.992 | 0.993 | 0.861 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 5.6 | 293 | Ammonium sulfate Sodium citrate Sodium thiocyanate Benzamidine Hydrochloride |
