6KUN

Crystal structure of dioxygenase for auxin oxidation (DAO) in rice

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-04-15
Detector	RAYONIX MX-225
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	75.247, 78.304, 94.003
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.307 - 2.002
R-factor	0.1905
Rwork	0.188
R-free	0.24270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ku3
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.000	2.000
Number of reflections	38070	2675
<I/σ(I)>	36.2
Completeness [%]	100.0
Redundancy	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Sodium acetate, 0.2 M ammonium sulfate and 22% (w/v) PEG 4000