6KUM
Ferredoxin I from C. reinhardtii, low X-ray dose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 25.878, 32.326, 49.915 |
| Unit cell angles | 72.38, 78.06, 72.39 |
Refinement procedure
| Resolution | 28.740 - 1.400 |
| R-factor | 0.1722 |
| Rwork | 0.171 |
| R-free | 0.18970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.435 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.5.26) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.740 | 28.740 | 1.420 |
| High resolution limit [Å] | 1.400 | 7.670 | 1.400 |
| Rmerge | 0.109 | 0.113 | 0.302 |
| Rmeas | 0.126 | 0.132 | 0.350 |
| Rpim | 0.064 | 0.068 | 0.177 |
| Total number of observations | 611 | 5108 | |
| Number of reflections | 54418 | 170 | 1328 |
| <I/σ(I)> | 7.9 | 11.7 | 3.8 |
| Completeness [%] | 95.7 | 96.6 | 93 |
| Redundancy | 3.8 | 3.6 | 3.8 |
| CC(1/2) | 0.987 | 0.966 | 0.917 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 5.6 | 298 | Ammonium Sulfate, Sodium Citrate, Benzamidine Hydrochloride, Sodium Thiocyanate |
