6KUI

Active conformation of HslV from Staphylococcus aureus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-10-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97960
Spacegroup name	I 2 2 2
Unit cell lengths	95.670, 108.776, 123.004
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.250 - 2.330
R-factor	0.2004
Rwork	0.196
R-free	0.25110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kr1
RMSD bond length	0.002
RMSD bond angle	0.460
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.370
High resolution limit [Å]	2.330	2.330
Rmeas	0.073	0.400
Rpim	0.021	0.184
Number of reflections	27546	1245
<I/σ(I)>	24.5	2.5
Completeness [%]	98.6
Redundancy	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	Bis-Tris propane, Citric acid, PEG 3350