6KUG

Crystal structure of YBX1 CSD with RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-25
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9789
Spacegroup name	P 62
Unit cell lengths	66.473, 66.473, 34.438
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	21.758 - 1.400
R-factor	0.1816
Rwork	0.178
R-free	0.21300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.689
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.450
High resolution limit [Å]	1.400	1.400
Rmerge		0.800
Number of reflections	17335	1715
<I/σ(I)>	44.4
Completeness [%]	100.0	100
Redundancy	19.2
CC(1/2)		0.912

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2.4 M Ammonium sulfate, 0.1M citric acid pH 5.0.