6KU6

OSM1 mutant - R326A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	104.15
Detector technology	PIXEL
Collection date	2016-06-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97950
Spacegroup name	P 1 21 1
Unit cell lengths	51.924, 109.924, 77.142
Unit cell angles	90.00, 98.52, 90.00

Refinement procedure

Resolution	38.145 - 2.007
R-factor	0.2013
Rwork	0.200
R-free	0.25050
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.145	2.030
High resolution limit [Å]	2.007	2.007
Number of reflections	56568	639
<I/σ(I)>	15.0625
Completeness [%]	99.9
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293.15	PEG 3350, BIS TRIS pH 5.5