6KU0
Crystal structure of MyoVa-GTD in complex with MICAL1-GTBM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-29 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.293, 63.220, 74.418 |
| Unit cell angles | 65.78, 86.71, 85.81 |
Refinement procedure
| Resolution | 50.000 - 1.600 |
| R-factor | 0.1619 |
| Rwork | 0.161 |
| R-free | 0.19890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wb8 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.709 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 4.340 | 1.600 |
| Rmerge | 0.056 | 0.049 | 0.418 |
| Rmeas | 0.063 | 0.055 | 0.485 |
| Rpim | 0.029 | 0.025 | 0.241 |
| Total number of observations | 542390 | ||
| Number of reflections | 121058 | 6152 | 5464 |
| <I/σ(I)> | 6.9 | ||
| Completeness [%] | 92.4 | 93.9 | 83.5 |
| Redundancy | 4.5 | 4.8 | 3.6 |
| CC(1/2) | 0.988 | 0.827 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 1% w/v Tryptone, 0.05M HEPES sodium pH 7.0, 20% w/v Polyethylene glycol 3350 |
