6KTY
Crystal structure of the flagellar cap protein FliD from Bdellovibrio bacteriovorus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-28 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 97.811, 97.811, 83.057 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.990 |
| R-factor | 0.21801 |
| Rwork | 0.216 |
| R-free | 0.25733 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.020 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.060 | 0.271 |
| Number of reflections | 13863 | 694 |
| <I/σ(I)> | 46.5 | 7.1 |
| Completeness [%] | 98.0 | 99.7 |
| Redundancy | 6.1 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | ammonium sulfate, MES, PEG 400 |
