6KTH

Crystal structure of Juvenile hormone diol kinase JHDK-L2 from silkworm, Bombyx mori

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	CCD
Collection date	2018-12-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	47.960, 67.570, 69.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.500 - 1.220
Rwork	0.183
R-free	0.20000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ccm
RMSD bond length	0.024
RMSD bond angle	2.467
Data reduction software	HKL-3000
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.484	1.250
High resolution limit [Å]	1.220	1.220
Rmerge	0.074	1.304
Number of reflections	67534
<I/σ(I)>	17.6	2.1
Completeness [%]	99.5	99.5
Redundancy	12.48	12.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	289	0.2M Potassium sodium tartrate tetrahydrate, 0.1M Sodium citrate tribasic dihyrate pH 5.6, 2 M Ammonium sulfate.