6KT1
Crystal structure of an OspA mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-08 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.074, 54.285, 66.190 |
| Unit cell angles | 90.00, 100.37, 90.00 |
Refinement procedure
| Resolution | 19.772 - 1.430 |
| Rwork | 0.177 |
| R-free | 0.20790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g8c |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.690 | 1.460 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.037 | 0.452 |
| Number of reflections | 42406 | 2225 |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 99.4 | 99.2 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 0.1 M Tris-HCl,40% PEG 400 |
