6KSY
Crystal structure of arginase from Zymomonas mobilis ZM4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97942 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.107, 100.324, 79.600 |
| Unit cell angles | 90.00, 113.31, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.649 |
| Rwork | 0.165 |
| R-free | 0.18770 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.680 |
| High resolution limit [Å] | 1.649 | 1.650 |
| Rpim | 0.036 | 0.388 |
| Number of reflections | 131592 | 6498 |
| <I/σ(I)> | 17.6 | |
| Completeness [%] | 95.2 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 25 % (w/v) Polyethylene glycol 3350, 0.2 M Lithium Sulfate, 0.1 M Tris-HCl at pH 8.5 |
