6KSN

Structure of a Zn-bound camelid single domain antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-03-07
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	106.880, 106.880, 70.580
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.280 - 2.150
R-factor	0.1952
Rwork	0.193
R-free	0.23240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3jbe
RMSD bond length	0.006
RMSD bond angle	0.914
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.220
High resolution limit [Å]	2.150	2.150
Rmerge	0.012	1.146
Rmeas	0.138	1.223
Rpim	0.045	0.422
Number of reflections	25502	2171
<I/σ(I)>	11.1	1.9
Completeness [%]	99.6	98.7
Redundancy	8.9	8
CC(1/2)	0.997	0.624

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.25	277	0.1M Tris, pH8.25, 3.5M Na Formate