6KSG

Vibrio cholerae Methionine Aminopeptidase in holo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9536
Spacegroup name	P 1 21 1
Unit cell lengths	49.508, 49.835, 131.221
Unit cell angles	90.00, 97.28, 90.00

Refinement procedure

Resolution	46.580 - 1.900
R-factor	0.1978
Rwork	0.196
R-free	0.23940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6k26
RMSD bond length	0.010
RMSD bond angle	1.542
Data reduction software	XDS
Data scaling software	Aimless (0.7.3)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.000	48.000	1.950
High resolution limit [Å]	1.900	8.920	1.900
Rmerge	0.074	0.036	0.795
Rmeas	0.081	0.040	0.873
Rpim	0.032	0.017	0.355
Total number of observations	307534	2841	18919
Number of reflections	50007	539	3247
<I/σ(I)>	15.8	39.9	2.7
Completeness [%]	99.6	99.1	97.8
Redundancy	6.1	5.3	5.8
CC(1/2)	0.999	0.999	0.874

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	PEG 3350