6KSG
Vibrio cholerae Methionine Aminopeptidase in holo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9536 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.508, 49.835, 131.221 |
| Unit cell angles | 90.00, 97.28, 90.00 |
Refinement procedure
| Resolution | 46.580 - 1.900 |
| R-factor | 0.1978 |
| Rwork | 0.196 |
| R-free | 0.23940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6k26 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.542 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.000 | 48.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 8.920 | 1.900 |
| Rmerge | 0.074 | 0.036 | 0.795 |
| Rmeas | 0.081 | 0.040 | 0.873 |
| Rpim | 0.032 | 0.017 | 0.355 |
| Total number of observations | 307534 | 2841 | 18919 |
| Number of reflections | 50007 | 539 | 3247 |
| <I/σ(I)> | 15.8 | 39.9 | 2.7 |
| Completeness [%] | 99.6 | 99.1 | 97.8 |
| Redundancy | 6.1 | 5.3 | 5.8 |
| CC(1/2) | 0.999 | 0.999 | 0.874 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 3350 |
