6KS5

Crystal structure of Legionella pneumophila deubiquitinase Ceg23

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-07-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979191
Spacegroup name	P 21 21 21
Unit cell lengths	67.350, 71.610, 142.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.441 - 2.800
R-factor	0.2748
Rwork	0.274
R-free	0.29900
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.020	2.870
High resolution limit [Å]	2.800	2.800
Rmerge	0.122
Rpim	0.036
Number of reflections	17475	1226
<I/σ(I)>	2
Completeness [%]	99.0	97
Redundancy	12.8
CC(1/2)	0.995	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	289	20% polyethylene glycol 4000, 0.2 M sodium cacodylate (pH 6.0) and 0.2 M NaCl