6KS2
Structure of anti-Ghrelin receptor antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-16 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.239, 107.916, 189.892 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.473 - 1.753 |
R-factor | 0.1942 |
Rwork | 0.192 |
R-free | 0.23280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1q9k |
RMSD bond length | 0.011 |
RMSD bond angle | 1.215 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (dev_2210) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.473 | 1.810 |
High resolution limit [Å] | 1.740 | 1.740 |
Rmerge | 0.101 | 1.196 |
Rpim | 0.062 | 0.740 |
Number of reflections | 169522 | 16885 |
<I/σ(I)> | 6.7 | |
Completeness [%] | 99.1 | 98.66 |
Redundancy | 3.53 | 3.54 |
CC(1/2) | 0.995 | 0.498 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100mM BICINE pH 9.0, 100mM Sodium Chloride, 30%(v/v) PEG550MME |