6KRS
Peroxiredoxin from Aeropyrum pernix K1 (ApPrx) 0Cys W211A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 |
| Unit cell lengths | 76.730, 103.530, 105.790 |
| Unit cell angles | 106.17, 104.50, 92.78 |
Refinement procedure
| Resolution | 49.363 - 2.300 |
| Rwork | 0.189 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e2g |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.053 | 0.265 |
| Rmeas | 0.059 | 0.295 |
| Number of reflections | 130548 | 6421 |
| <I/σ(I)> | 17 | 5.5 |
| Completeness [%] | 97.9 | 97.5 |
| Redundancy | 5.3 | 5.3 |
| CC(1/2) | 0.998 | 0.982 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 1.0 M Ammonium phosphate monobasic |
