6KRN
Crystal structure of GH30 xylanase B from Talaromyces cellulolyticus expressed by Pichia pastoris in complex with aldotriuronic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 173 |
Detector technology | PIXEL |
Collection date | 2019-06-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.251, 78.700, 118.418 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.410 - 1.653 |
Rwork | 0.170 |
R-free | 0.18970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6iuj |
RMSD bond length | 0.015 |
RMSD bond angle | 1.704 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP (11.6) |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.410 | 1.712 |
High resolution limit [Å] | 1.653 | 1.653 |
Rmerge | 0.046 | 0.458 |
Rmeas | 0.053 | 0.518 |
Rpim | 0.024 | 0.239 |
Number of reflections | 71087 | 7017 |
<I/σ(I)> | 15.35 | 2.34 |
Completeness [%] | 99.4 | 99.03 |
Redundancy | 4.5 | 4.5 |
CC(1/2) | 0.999 | 0.847 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 293 | PEG 3350, HEPES, magnesium chloride |