6KRG

Crystal structure of sfGFP Y182TMSiPhe

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-21
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97927
Spacegroup name	I 41
Unit cell lengths	68.565, 68.565, 119.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.720 - 1.400
R-factor	0.1494
Rwork	0.148
R-free	0.17320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jfg
RMSD bond length	0.006
RMSD bond angle	0.916
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.990	1.420
High resolution limit [Å]	1.399	1.400
Rmerge	0.048	0.438
Rmeas	0.053	0.192
Rpim	0.021	0.479
Number of reflections	53860	2698
<I/σ(I)>	32.4	2.78
Completeness [%]	99.7	99.9
Redundancy	6.2	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	PEG 6000, SODIUM CHLORIDE