6KQS
Crystal Structure of GH136 lacto-N-biosidase from Eubacterium ramulus - selenomethionine derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-09 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 132.734, 132.734, 82.466 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.750 - 1.400 |
| R-factor | 0.1498 |
| Rwork | 0.149 |
| R-free | 0.17240 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.975 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.750 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.145 | 1.909 |
| Number of reflections | 155798 | 8031 |
| <I/σ(I)> | 12.2 | 1.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20.1 | 19.4 |
| CC(1/2) | 0.999 | 0.728 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 20% PEG 6000, 0.1 M Tris-HCl (pH 8.5), 1 M LiCl |
