6KQL
Thermus thermophilus initial transcription complex comprising sigma A and 5'-triphosphate RNA of 4 nt
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-08 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.075 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 183.802, 103.166, 295.560 |
| Unit cell angles | 90.00, 99.12, 90.00 |
Refinement procedure
| Resolution | 44.844 - 2.890 |
| R-factor | 0.208 |
| Rwork | 0.207 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g7h |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.040 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.000 | 45.000 | 2.930 |
| High resolution limit [Å] | 2.880 | 7.800 | 2.880 |
| Rmerge | 0.110 | 0.076 | 0.489 |
| Total number of observations | 450274 | ||
| Number of reflections | 121378 | 6249 | 6033 |
| <I/σ(I)> | 10.8 | ||
| Completeness [%] | 99.9 | 99.5 | 99.1 |
| Redundancy | 3.7 | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.05 M Magnesium chloride, 0.2 M Potassium chloride, 0.1 M Tris-HCl pH8.0, 10% PEG 4000 |
