6KQG
Thermus thermophilus initial transcription complex comprising sigma A and 5'-OH RNA of 6 nt
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-30 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 184.236, 104.037, 296.845 |
| Unit cell angles | 90.00, 98.65, 90.00 |
Refinement procedure
| Resolution | 49.029 - 2.783 |
| R-factor | 0.207 |
| Rwork | 0.205 |
| R-free | 0.24790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g7h |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
| High resolution limit [Å] | 2.783 | 7.590 | 2.800 |
| Rmerge | 0.074 | 0.036 | 0.710 |
| Number of reflections | 137078 | 7067 | 6666 |
| <I/σ(I)> | 7.9 | ||
| Completeness [%] | 99.8 | 99.9 | 98 |
| Redundancy | 3.4 | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.05 M Magnesium chloride, 0.2 M Potassium chloride, 0.1 M Tris-HCl pH8.0, 10% PEG 4000 |
