6KQ1

Crystal structure of cytochrome c551 from Pseudomonas sp. strain MT-1.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-17
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	50.787, 52.017, 62.393
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.978 - 1.570
R-factor	0.2031
Rwork	0.201
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	351c
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.16-3549-000)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.950	39.950	1.600
High resolution limit [Å]	1.570	8.600	1.570
Rmerge	0.095	0.040	1.188
Total number of observations	309252	1428	13155
Number of reflections	23763	183	1168
<I/σ(I)>	15	37.3	2.1
Completeness [%]	100.0	99.4	99.9
Redundancy	13	7.8	11.3
CC(1/2)	0.999	0.999	0.918

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	10% (w/v) PEG 8000, 100 mM MES/ Sodium hydroxide pH 6.0, 200 mM Zinc acetate