6KP3
STRUCTURE OF SENDAI VIRUS Y3/ALIX-BRO1 DOMAIN COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.327, 102.648, 103.629 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.310 - 2.200 |
| R-factor | 0.2079 |
| Rwork | 0.205 |
| R-free | 0.26170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oew |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.478 |
| Data reduction software | TRUNCATE (1.17.29) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP (11.7.02) |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.260 | 46.260 | 2.270 |
| High resolution limit [Å] | 2.200 | 9.070 | 2.200 |
| Rmerge | 0.062 | 0.032 | 0.880 |
| Rmeas | 0.069 | 0.037 | 0.974 |
| Rpim | 0.030 | 0.017 | 0.413 |
| Total number of observations | 151908 | 2208 | 13156 |
| Number of reflections | 28045 | 476 | 2383 |
| <I/σ(I)> | 16.9 | 54 | 2.1 |
| Completeness [%] | 100.0 | 99.7 | 100 |
| Redundancy | 5.4 | 4.6 | 5.5 |
| CC(1/2) | 0.998 | 0.996 | 0.843 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.45 | 297 | 15% PEG 3350, 0.2M Sodium Malonate-NaOH |
