6KOR

Crystal structure of the RRM domain of SYNCRIP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	193.15
Detector technology	CCD
Collection date	2018-04-06
Detector	RIGAKU
Wavelength(s)	1.54187
Spacegroup name	H 3
Unit cell lengths	103.634, 103.634, 49.289
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.908 - 2.602
R-factor	0.1855
Rwork	0.182
R-free	0.24990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dgu
RMSD bond length	0.010
RMSD bond angle	1.146
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CRANK2
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.908	2.640
High resolution limit [Å]	2.602	2.602
Rmerge		0.049
Number of reflections	6026	6026
<I/σ(I)>	11.2
Completeness [%]	99.7
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1M HEPES-NaOH (pH 7.5), 20% PEG 10,000