6KOK

Crystal Structure of SNX11/SNX10-PXe Chimera

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-19
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9789
Spacegroup name	P 1 21 1
Unit cell lengths	40.103, 47.910, 87.309
Unit cell angles	90.00, 94.39, 90.00

Refinement procedure

Resolution	43.530 - 2.000
R-factor	0.2065
Rwork	0.204
R-free	0.24970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ikb
Data reduction software	MOSFLM
Data scaling software	Aimless (0.6.2)
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.910	47.910	2.050
High resolution limit [Å]	2.000	8.950	2.000
Rmerge	0.073	0.059	0.125
Rmeas	0.085	0.069	0.147
Rpim	0.044	0.036	0.075
Total number of observations		1027	5700
Number of reflections	21816	280	1585
<I/σ(I)>	11.7	15.7	7.6
Completeness [%]	97.0	99	97.6
Redundancy	3.6	3.7	3.6
CC(1/2)	0.993	0.987	0.971

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.6	293	2.2 M NaCl, 0.1 M NaAc pH 4.6