6KO6

Crystal structure of AMPPNP bound Cka1 from C. neoformans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-27
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.97
Spacegroup name	C 2 2 21
Unit cell lengths	89.020, 95.800, 93.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.670 - 2.400
Rwork	0.238
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lp4
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14-3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.670	2.486
High resolution limit [Å]	2.400	2.400
Rmerge	0.042	0.286
Number of reflections	15949	1554
<I/σ(I)>	10.6	2.35
Completeness [%]	99.5
Redundancy	2
CC(1/2)	0.998	0.929

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	290	0.2M lithium sulfate, 0.1M Tris pH 8.5, 30%(w/v) PEG 4000, 30%(w/v) dextran sulfate sodium salt, 5mM AMPPNP, 5mM MgCl2