6KNE

The C-terminal Domain of Translation Initiation Factor 5 at high pH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-21
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	49.950, 54.156, 63.285
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.147 - 1.701
R-factor	0.2319
Rwork	0.230
R-free	0.26530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.985
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.147	41.147	1.800
High resolution limit [Å]	1.700	5.070	1.700
Rmerge	0.031	0.022	0.508
Rmeas	0.035	0.026	0.574
Total number of observations	87296
Number of reflections	19254	805	3068
<I/σ(I)>	19.98	50.54	2.71
Completeness [%]	99.0	97.5	99.3
Redundancy	4.534	4.184	4.55
CC(1/2)	0.999	0.999	0.971

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	sodium citrate pH 5.5, 40% (v/v) PEG 600