6KN9
Crystal structure of human interleukin 18 receptor beta extracellular domain in complex with an antagonistic scFv
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-07-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97775 |
| Spacegroup name | P 31 |
| Unit cell lengths | 163.160, 163.160, 64.145 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.190 - 3.302 |
| R-factor | 0.2484 |
| Rwork | 0.247 |
| R-free | 0.27690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wo4 3pnw |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.376 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 7.100 | 3.300 |
| Rmerge | 0.113 | 0.061 | 0.652 |
| Rmeas | 0.134 | 0.075 | 0.796 |
| Rpim | 0.071 | 0.042 | 0.450 |
| Total number of observations | 96462 | ||
| Number of reflections | 28309 | 2807 | 2743 |
| <I/σ(I)> | 7.7 | ||
| Completeness [%] | 98.6 | 97.5 | 95.3 |
| Redundancy | 3.4 | 3.3 | 3 |
| CC(1/2) | 0.988 | 0.558 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 288 | 0.2M Ammonium iodide, 20% polyethylene glycol 3350 |
