6KN1
P20/P12 of caspase-11 mutant C254A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2019-03-29 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.001, 99.941, 58.294 |
Unit cell angles | 90.00, 114.38, 90.00 |
Refinement procedure
Resolution | 46.890 - 1.900 |
R-factor | 0.189 |
Rwork | 0.188 |
R-free | 0.21750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6kmu |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.890 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.064 | 0.291 |
Number of reflections | 42565 | 3036 |
<I/σ(I)> | 14.2 | 3.93 |
Completeness [%] | 98.9 | 95.1 |
Redundancy | 3.47 | |
CC(1/2) | 0.996 | 0.941 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M sodium citrate pH 5.7 and 18% (w/v) polyethylene glycol 3350 |