6KME
Crystal structure of phytochromobilin synthase from tomato in complex with biliverdin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-30 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 64.130, 64.130, 158.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.851 - 1.950 |
R-factor | 0.1646 |
Rwork | 0.162 |
R-free | 0.20650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6kmd |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (v1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.851 | 49.851 | 2.060 |
High resolution limit [Å] | 1.950 | 5.770 | 1.950 |
Rmerge | 0.534 | 0.184 | 4.222 |
Rmeas | 0.537 | 0.185 | 4.246 |
Rpim | 0.055 | 0.447 | |
Number of reflections | 25049 | 1124 | 3719 |
<I/σ(I)> | 17.6 | 43.65 | 3.81 |
Completeness [%] | 100.0 | 99.6 | 100 |
Redundancy | 95.059 | 93.854 | 91.257 |
CC(1/2) | 0.997 | 0.998 | 0.959 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | PEG 8000, magnesium chloride, Tris-HCl |