6KLJ
Crystal Structure of the Zea Mays laccase 3 complexed with coniferyl
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-15 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 111.848, 111.848, 208.185 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.944 - 1.998 |
R-factor | 0.1661 |
Rwork | 0.164 |
R-free | 0.20500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1aoz |
RMSD bond length | 0.007 |
RMSD bond angle | 0.889 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.030 |
High resolution limit [Å] | 1.998 | 1.998 |
Rmerge | 0.097 | 0.447 |
Number of reflections | 52763 | 2573 |
<I/σ(I)> | 32 | 6.3 |
Completeness [%] | 99.9 | |
Redundancy | 15.3 | |
CC(1/2) | 0.999 | 0.967 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 15% of PEG 3350, 0.2 M (NH4)2SO4 and 0.1 M Bis-tris,pH 5.5 |