6KLF
Crystal structure of branching enzyme D434A mutant from Cyanothece sp. ATCC 51142
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-25 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 133.745, 133.745, 184.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.290 - 2.500 |
| R-factor | 0.15503 |
| Rwork | 0.153 |
| R-free | 0.19524 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gqx |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.118 | 0.365 |
| Number of reflections | 58556 | 2880 |
| <I/σ(I)> | 18.8 | |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 10.8 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 8%(w/v) ethanol, 0.1 M HEPES-NaOH pH 7.2, 0.2 M MgCl2 |
