6KKN

Crystal structure of RuBisCO accumulation factor Raf1 from Anabaena sp. PCC 7120

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.978530
Spacegroup name	P 32 2 1
Unit cell lengths	94.740, 94.740, 117.980
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.850
R-factor	0.2274
Rwork	0.226
R-free	0.26030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wt3 4wt4
RMSD bond length	0.005
RMSD bond angle	1.081
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.020
High resolution limit [Å]	2.850	2.850
Rmerge	0.085	0.760
Number of reflections	14798	2325
<I/σ(I)>	18.3
Completeness [%]	99.9
Redundancy	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289.15	2.4M Sodium acetate trihydrate