6KKM
Crystal structure of RbcL-Raf1 complex from Anabaena sp. PCC 7120
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-19 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97892 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 222.117, 228.541, 162.336 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.000 - 3.000 |
| R-factor | 0.2312 |
| Rwork | 0.229 |
| R-free | 0.27810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rbl |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.195 | 1.095 |
| Number of reflections | 82605 | 8166 |
| <I/σ(I)> | 16 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 8-10% 2-Methyl-2,4-pentanediol and 0.1 M MES 6.0 |
