6KKG

Crystal structure of MAGI2-Dendrin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91877
Spacegroup name	P 32
Unit cell lengths	55.715, 55.715, 74.696
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.125 - 2.150
R-factor	0.2049
Rwork	0.202
R-free	0.26350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ysd 2yse
RMSD bond length	0.008
RMSD bond angle	1.180
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.190
High resolution limit [Å]	2.150	5.830	2.150
Rmerge	0.082	0.071	0.455
Rmeas	0.095	0.082	0.528
Rpim	0.047	0.039	0.265
Number of reflections	13900	705	684
<I/σ(I)>	7.2
Completeness [%]	98.6	99.2	100
Redundancy	4.2	4.2	4
CC(1/2)		0.988	0.856

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	2.2M Ammonium Sulfate, 150mM Malate at pH 5.1, 4.0% v/v 1,2-Propanedio