6KK9
A Crystal structure of OspA mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.537, 138.641, 62.514 |
| Unit cell angles | 90.00, 100.88, 90.00 |
Refinement procedure
| Resolution | 19.900 - 2.200 |
| R-factor | 0.225655815502 |
| Rwork | 0.222 |
| R-free | 0.28685 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cka |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.029 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.068 | 0.470 |
| Number of reflections | 43410 | 4307 |
| <I/σ(I)> | 30.1 | 3.44 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 7.2 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 30% PEG 400, 0.1 M Tris-HCl |
