6KJK
Structure of the N-terminal domain of PorA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 67.300, 43.220, 48.590 |
| Unit cell angles | 90.00, 108.52, 90.00 |
Refinement procedure
| Resolution | 46.100 - 1.300 |
| R-factor | 0.1821 |
| Rwork | 0.181 |
| R-free | 0.20300 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.057 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.100 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.038 | 0.329 |
| Number of reflections | 31131 | 1548 |
| <I/σ(I)> | 17.9 | 3.9 |
| Completeness [%] | 95.5 | 95.6 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 293 | 0.1M (NH4)2SO4, 26%(w/v) PEG-8000 |
