6KIS

High-resolution structure of mouse CXorf40A (soaked with Methylmercury chloride)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00928
Spacegroup name	P 21 21 2
Unit cell lengths	51.162, 95.784, 33.246
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.540
R-factor	0.2078
Rwork	0.206
R-free	0.24570
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXDE
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.600
High resolution limit [Å]	1.540	1.540
Number of reflections	24850	2461
<I/σ(I)>	36.57
Completeness [%]	99.5
Redundancy	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M HEPES sodium pH 7.5, 2% v/v Polyethylene glycol 400, 2.0 M Ammonium sulfate