6KIR

High resolution structure of mouse CXorf40A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97736
Spacegroup name	P 2 21 21
Unit cell lengths	33.252, 50.943, 96.292
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.860 - 1.550
Rwork	0.181
R-free	0.24700
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.610
High resolution limit [Å]	1.550	1.550
Number of reflections	24456	2333
<I/σ(I)>	38.9
Completeness [%]	99.7
Redundancy	21.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2.0 M Ammonium sulfate, 2% Polyethylene glycol 400, 0.1 M Hepes pH7.5