6KIM

Crystal structure of diamondback moth ryanodine receptor SPRY2 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	193.15
Detector technology	PIXEL
Collection date	2019-06-13
Detector	DECTRIS PILATUS3 R CdTe 300K
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	68.025, 37.637, 67.476
Unit cell angles	90.00, 93.24, 90.00

Refinement procedure

Resolution	29.903 - 2.057
R-factor	0.1928
Rwork	0.186
R-free	0.25130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4p9j
Data reduction software	HKL-3000
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3247)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.903	2.166
High resolution limit [Å]	2.057	2.057
Rmerge	0.094	0.400
Number of reflections	10749	162
<I/σ(I)>	11.3
Completeness [%]	98.9
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	2.0 M Sodium/Potassium phosphate, Ph 6.5.