6KHT

Chimeric beta-glucosidase Cel1b-H13

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9781
Spacegroup name	C 2 2 21
Unit cell lengths	108.580, 182.393, 210.507
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.234 - 3.305
R-factor	0.1773
Rwork	0.174
R-free	0.23690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nlr
RMSD bond length	0.011
RMSD bond angle	1.566
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.360
High resolution limit [Å]	3.300	3.300
Number of reflections	31640	1550
<I/σ(I)>	12.89
Completeness [%]	100.0
Redundancy	12.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.5	289	2.0 M Ammonium sulfate, 0.1 M Bis-Tris pH 5.5, 20% (vol/vol) glycerol