6KHM
Lipase (Open form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-04 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 31 |
| Unit cell lengths | 185.857, 185.857, 205.099 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.640 - 2.400 |
| R-factor | 0.1881 |
| Rwork | 0.185 |
| R-free | 0.25570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6khk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.686 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.110 | 0.480 |
| Rpim | 0.040 | 0.180 |
| Number of reflections | 310189 | 15265 |
| <I/σ(I)> | 19 | |
| Completeness [%] | 97.6 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 297 | 2M (NH4)2SO4, 0.2M MgCl2, and 0.1M Bis-Tris |
