6KGI
RLGS-yUbr1 Ubr box
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2018-05-04 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 60.630, 60.630, 69.811 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.250 - 1.040 |
| R-factor | 0.1898 |
| Rwork | 0.189 |
| R-free | 0.20180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nit |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.060 |
| High resolution limit [Å] | 1.040 | 1.040 |
| Number of reflections | 62982 | 6200 |
| <I/σ(I)> | 43.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M BIS-Tris pH 6.5, 2.7-3.0M NaCl |
