6KG6

Crystal structure of MavC/UBE2N-Ub complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	107.352, 97.185, 74.376
Unit cell angles	90.00, 103.27, 90.00

Refinement procedure

Resolution	30.940 - 2.390
R-factor	0.1923
Rwork	0.190
R-free	0.24480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tsc 1j7d 1ubq
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.13-2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.480
High resolution limit [Å]	2.390	2.390
Rmerge	0.072	0.325
Number of reflections	29359	2853
<I/σ(I)>	22.6
Completeness [%]	99.6
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	PEG 4000, Tris